Skillsets
MOLECULAR BIOLOGY EXPERTISE
Molecular Biology Techniques
Vector designing, construction
Cloning- recombinant DNA technology
Basic Microbiology and Cell and Tissue Culture techniques
Protein Engineering
PCR (qPCR, ddPCR)
Western Blotting, ELISA
Electroporation, Gene delivery
IMMUNOLOGY-CELL BIOLOGY-VIROLOGY
Immunological Assays Cell-sorting by FACS
Flow Cytometry
Immunohistochemistry
Purification and Protein Crystallization of RNAvirusesÂ
DNA Encoded Library affinity Selection for RNA helicases
RNA seq, Next Generation Sequencing (NGS)
BIOPHYSICAL & BIOCHEMICAL TECHNIQUES
Expressing protein from bacteria (E.coli), insect cell line (sf9, baculoviruses)
Purification protein with AKTA, HPLC, SEC, IEX, RP
Proteasome activation, Pull down assays
TR-FRET Assay, Mass Spectrometry, LC-MS
Analytical Ultracentrifugation
SDS-PAGE
STRUCTURAL BIOLOGY TECHNIQUES
X-ray Crystallography
X-ray Scattering
Neutron Scattering
Nuclear Magnetic Resonance (NMR)
Cryo-EM Microscopy (Negative Staining)
High Speed Atomic Force Field Microscopy
Dynamic Light Scattering (DLS)
Grated Coupling Interferometry (GCI)
Surface Plasmon Resonance (SPR) Biacore8K
ITC High throughput screening (HTS)
BIOINFORMATICS/COMPUTATIONAL BIOLOGY SKILLS
Programming ----C, JAVA, PERL, PYTHON
Databases---MySQL, PL/ SQL (Oracle 9i)
MATLAB, SPSS, GraphPad Prism, R
BLAST, MATLAB, SWISSPDBViwer, AUTODOCK
Coot, RefMac, PDBePISA,Schrodinger, PYMOL, KNIME, Spotfire
Transcriptomics Software-- Vis Ant,Mockiview, Cytoscape, GeneSpring, GEODATABASE (Gene Ontology Annotation), ARRAY EXPRESS, Gene Cards3, PIPs,STRING8.3, GOLD3.0